Comparación de métodos para la evaluación de la calidad del ADN
DOI:
https://doi.org/10.70833/rseisa17item369Keywords:
Migración, Trabajo, Género, Discriminación, CuidadoAbstract
La extracción de ADN es el primer paso crucial para llevar a cabo análisis genéticos en una amplia variedad de áreas de investigación. Aunque se puede utilizar ADN de calidad y cantidad subóptimas según la finalidad, durante el proceso de extracción se deben evaluar estos parámetros para optimizar el método y su eficiencia. Con estos factores en mente, el objetivo de este estudio fue comparar la eficacia de la electroforesis en gel de agarosa y la espectrofotometría de micro volumen para evaluar la calidad del ADN de ocho hierbas medicinales, una fresca y siete secas extraído utilizando la técnica de CTAB con modificaciones. Se determinó la concentración de ADN mediante el uso de un espectrofotómetro de micro volumen y se realizó una corrida en gel de agarosa al 0.8%. Los resultados obtenidos fueron comparados y los resultados obtenidos por espectrofotometría de micro volumen y geles de agarosa no fueron coincidentes siendo visualizado únicamente el ADN de la hierba medicinal en estado fresco.
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Copyright (c) 2022 Andrea Alejandra Arrúa Widmer, Martín Concepción Núñez Giménez , Nadia Ortiz, Laura Santander Dïaz, Rocío De las Nieves Coronel Cristaldo, Lourdes Cardozo, Andrea Guadalupe Torres Nunes, Celso Duarte, Moisés Rojas, Liz Camila Quiñonez Alvarez, Gilberto Antonio Benítez Rodas, Danilo Fernández Ríos
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